2-Amino-2-methyl-1-propanol as the Alkalizing Agent in an Improved Continuous-Flow Cresolphthalein Complexone Procedure for Calcium in Serum
- 1 November 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 20 (11) , 1458-1460
- https://doi.org/10.1093/clinchem/20.11.1458
Abstract
We describe an improved cresolphthalein complexone procedure for calcium in which diethylamine is replaced by a nontoxic amino alcohol. Advantages of the procedure are increased sensitivity, excellent baseline stability, a more optimum reaction environment (pH 10.0) that eliminates blanking problems, and freedom from interference by magnesium. Values obtained by this method are not significantly different (P < 0.05) from those obtained by atomic absorption spectrophotometry.Keywords
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