Electron Microscopy of Purified Lansing Virus.

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Abstract
Purified prepns. of Lansing virus of specific activities, I.D.50, from 10-10.1 to 10-8.3 g. of virus N revealed spherical or slightly asymmetrical particles when viewed in the Stanford electron microscope. Micrographs of nonshadowed specimens showed particles with an average width of 16 m[mu] and an average particle diam. of 23 m[mu]. Micro-graphs of prepns. treated by the gold shadowing technique disclosed a range of particle size as measured by shadow width from 15 to 34 m[mu] with an av. particle diam of 25 m[mu]. The latter value is considered the more accurate since the high degree of photographic contrast due to gold shadowing permits accurate measurement of the shadow width of the partides.

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