Métabolisme intracellulaire et extracellulaire de l'acide 3-indolylacétique dans les cultures aseptiques ou non aseptiques de pointes de racine du Pisum sativum

1 August 1968

journal article
Published by Canadian Science Publishing in Canadian Journal of Botany

Vol. 46 (8) , 969-978
https://doi.org/10.1139/b68-129

Abstract

Studies on indoleacetic acid (IAA) metabolism with root tips of 3-day-old pea seedlings were carried out by thin-layer chromatography and ¹⁴C-labelled auxin of high specific activity at 10⁻⁶ M. After 16-h incubation, five metabolites of IAA could be recognized in the tissues and two in solution; one metabolite was identified as o-formamidoacetophenone from its chromatographic R_f and ultraviolet absorption spectrum. Under sterile conditions the rate of breakdown of IAA is higher in the medium than under nonsterile conditions (even after removal of the tissues). We noted, too, a decrease of the action of IAA on the growth. The decreased activity of solution under nonsterile conditions is believed to be due to the production of oxidase inhibition by microorganisms.

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