ENHANCEMENT OF RETROVIRUS PRODUCTION BY ANTI-INTERFERON SERUM

  • 1 January 1979
    • journal article
    • research article
    • Vol. B130  (3) , 349-+
Abstract
To investigate the role of endogenous interferon in retrovirus production by infected or induced cells, the effect of 2 sera raised against mouse interferon is tested on various C-type murine viruses. Addition of a highly potent anti-interferon serum to 3TE/IC cells chronically infected by the Moloney strain of MLV [murine leukemia virus] results in a considerable increase of virus production as tested by reverse transcriptase assay. This effect is neutralized by excess exogenous interferon. The greatest effect of anti-interferon sera was obtained in the derepression of endogenous retroviruses: in K. BALB/c [kirsten sarcoma virus-transformed] cells treated by IUDR [iododeoxyuridine], anti-interferon serum increases up to 50-fold the expression of the endogenous virus. The extinction of virus production which secondarily occurs after its induction by IUdR may be caused by cellular endogenous interferon. The biological parameters of the viral agent produced in the presence of anti-interferon serum are those of the xenotropic endogenous virus.

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