CALCIUM- AND LIPID-DEPENDENT PROTEIN PHOSPHORYLATION IN THE HUMAN OVARY
- 1 October 1983
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 57 (4) , 872-874
- https://doi.org/10.1210/jcem-57-4-872
Abstract
The cytosol of human ovarian tissues was observed to promote protein phosphorylation in the combined presence of Ca2+,1,2-diolein, and phosphatidylserine. Ca2+alone or lipid alone did not produce full activation of this protein kinase(s). The addition of human erythrocyte calmodulin to the assay mixture, in the presence or absence of Ca2+, had no effect on protein kinase activity. Phosphorylation of cytosol proteins ranging in mol wt from 10,000 to 200,000 was selectively increased by Ca2+ plus lipid. This protein kinase activity may play a crucial role in the intracellular transmission of the action of hormones affecting cellular Ca2+ flux and/or phospholipid metabolism.Keywords
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