Modification of the Official Fluorometric Method for Selenium in Plants

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Abstract
The official first action AOAC fluorometric method for selenium in plants, 3.074–3.078, has been modified to simplify the method and to make it more accurate. The digestion time has been increased from 15 to 30 min past the appearance of perchloric acid fumes to better assure complete oxidation of all forms of selenium to selenite. Preparation of the 2,3-diaminonaphthalene solution, the reagent used for fluorometric analysis, has been changed so that the reagent is stable for several weeks; in the previous writeup, this solution had to be prepared daily. Special equipment (micro-Kjeldahl flasks with ground glass joints) has been eliminated and cyclohexane has been substituted for decahydronaphthalene. The modified method is convenient and applicable to a wide range of materials; it yields results comparable to those from the official method.

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