The bioassay of gibberellins
- 1 January 1966
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 72 (2) , 155-161
- https://doi.org/10.1007/bf00387479
Abstract
A bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: 1) Release of α-amylase is one step closer to the primary site of action of GA; 2) release of α-amylase is not affected by solvent residues and is apparently completely specific for gibberellin; 3) release of α-amylase is not affected by substances other than gibberellins present in crude plant extracts.Keywords
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