Determination of the structure of [Nle7]‐endothelin by 1H NMR
- 1 April 1991
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 37 (4) , 315-324
- https://doi.org/10.1111/j.1399-3011.1991.tb00745.x
Abstract
[Nle7]-endothelin was synthesized and studied by 1H NMR and distance geometry calculations. The NMR study was performed first in DMSO-d6 and then in 50% acetonitrile/water since this peptide aggregates in pure water. In both cases, all spin systems were identified and assigned with the aid of two-dimensional spectroscopy (2D): COSY (for scalar couplings) and NOESY (for dipolar couplings). On the basis of the acetonitrile/water NMR parameters, and using the DISGEO program, a three-dimensional structure of [Nle7]-endothelin is proposed and discussed.Keywords
This publication has 39 references indexed in Scilit:
- Different pathways of endothelin/sarafotoxin-stimulated phosphoinositide hydrolysis in myocytesEuropean Journal of Pharmacology: Molecular Pharmacology, 1990
- Free calcium rise and mitogenesis in glial cells caused by endothelinBiochemical and Biophysical Research Communications, 1989
- SRTX‐d, a new native peptide of the endothelin/sarafotoxin familyFEBS Letters, 1989
- Structure-activity relationship of endothelin: Importance of charged groupsBiochemical and Biophysical Research Communications, 1989
- Endothelin-1 induces vasoconstriction through two functionally distinct pathways in porcine coronary artery: Contribution of phosphoinositide turnoverBiochemical and Biophysical Research Communications, 1989
- Endothelin stimulates c‐fos and c‐myc expression and proliferation of vascular smooth muscle cellsFEBS Letters, 1988
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Two-dimensional relayed coherence transfer spectroscopy of a proteinJournal of Magnetic Resonance (1969), 1983
- Pseudo-structures for the 20 common amino acids for use in studies of protein conformations by measurements of intramolecular proton-proton distance constraints with nuclear magnetic resonanceJournal of Molecular Biology, 1983
- Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteinsBiochemical and Biophysical Research Communications, 1983