Structure-Function Analysis of Escherichia coli MnmG (GidA), a Highly Conserved tRNA-Modifying Enzyme
Open Access
- 15 December 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 191 (24) , 7614-7619
- https://doi.org/10.1128/jb.00650-09
Abstract
The MnmE-MnmG complex is involved in tRNA modification. We have determined the crystal structure of Escherichia coli MnmG at 2.4-Å resolution, mutated highly conserved residues with putative roles in flavin adenine dinucleotide (FAD) or tRNA binding and MnmE interaction, and analyzed the effects of these mutations in vivo and in vitro. Limited trypsinolysis of MnmG suggests significant conformational changes upon FAD binding.This publication has 9 references indexed in Scilit:
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