Quantitative Over-Pressure Layer Chromatography of Quinine, Quinidine, and Hydroquinidinein Biomedical Analysis

Abstract
An over-pressure layer chromatography (OPLC) method for the separation and quantification of quinine, quinidine and hydroquinidine is discussed in this paper. After the development with the mobile phase méthanol-acétonitrile-ammonia 0,2M (130: 68 : 2, v/v), a densitometric detection was performed by the fluorescence emission (excitation wavelength = 348 nm; emission wavelengh = 420 nm). This technique is simple, fast and adapted to small series of determination for the quality control and therapeutic drug monitoring.