Giemsa Staining of Mitotic Chromosomes in Kluyveromyces lactis and Saccharomyces cerevisiae

Abstract
A rapid and simple method for the assessment of chromosome numbers in yeast species would be most useful for taxonomic studies and where hybridization between different species and genera is contemplated. Previous success with air-drying and Giemsa-staining techniques for cytological examination of a simple eukaryote Dictyostelium discoideum suggested investigation of this method for the examination and possibly for the identification of yeast chromosomes. Hybrids are being constructed between K. lactis and S. cerevisiae. This report details studies on the chromosomes of these species which were undertaken to provide a cytological basis, in addition to genetic markers, for differentiating the 2 spp. and their hybrids.

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