Chromatography of Amino Acids on Reversed Phase Thin Layer Plates

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Abstract
The separation of 19 amino acids was studied on reversed phase thin layers, including C18 chemically bonded silica gel, impregnated silica gel, and acetylated cellulose. Normal aqueous-organic solvents, aqueous micellar solutions, and reversed micellar solutions were tested as mobile phases. The only practical system that provided a reversal in migration sequence compared to silica gel and cellulose included a C18 layer impregnated with HDBS, and this reversal was apparently due to an ion exchange mechanism.