Organic and inorganic nitrogen source ratio effects onBacillus thuringiensis var.israelensis delta-endotoxin production

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Abstract
The influence of different organic and inorganic nitrogen source combinations and C∶N ratios was studied in connection with growth and protein production ofBacillus thuringiensis var.israelensis. Protein production was assumed to be proportional to delta-endotoxin production. Delta-endotoxin concentration increased when media were supplemented with (NH4)2SO4, but the delta-endotoxin: biomass dry weight ratio was unaffected by different C∶N ratios. Organic nitrogen source, yeast extract, could be partially replaced by (NH4)2SO4 with a significant increase in delta-endotoxin production. On a étudié l'influence de diverses sources d'azote organique et inorganique et des rapports C∶N en relation avec la croissance et la production de protéine parBacillus thuringiensis var.israelensis. On fait l'hypothèse que la production de protéines est proportionnelle à la production de delta-endotoxine. La concentration de delta-endotoxine croît quand on ajoute au milieu du (NH4)2SO4, mais le rapport delta-endotoxine: poids sec de biomasse n'est pas affecté par différents rapports C∶N. On peut remplacer partiellement la source organique d'azote, l'extrait de levure, par du (NH4)2SO4 avec une augmentation significative de production de delta-endotoxine.

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