Inactivation of Slow Reacting Substance of Anaphylaxis (SRS-A) by Arylsulfatases
Open Access
- 1 July 1974
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 113 (1) , 316-322
- https://doi.org/10.4049/jimmunol.113.1.316
Abstract
Two different arylsulfatase preparations inactivated slow reacting substance of anaphylaxis (SRS-A) in a dose-related fashion. The loss of SRS-A activity could not be attributed to β-glucuronidase activity in the arylsulfatase preparations or to nonspecific adsorption to proteins. The arylsulfatase and SRS-A inactivating activities shared similar pH requirements, kinetics, and elution properties on Sephadex G-200 gel filtration. With spark source mass spectrometry, analysis of highly purified preparations of SRS-A revealed an abundance of 32S and 40Ca in the active samples as compared to controls. These findings indicate that SRS-A may contain a sulfate ester group.Keywords
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