Studies on the Specificity of Human Cytotoxic Antibody Reactive With Cultures of Lymphoid Cells

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Abstract
Studies are reported on an antigen of human cells demonstrated by reactions with a cytotoxic antibody in human serum. Quantitative determination of the antigen was performed by absorption of antibody-containing serum before reaction with the human lymphoid tissue culture cells. The absorption studies indicated that the antigen was present on all of 19 human lymphoid cell lines established from patients with a variety of diseases and from normal adults. Cell lines derived from patients with Burkitt's tumor contained the highest quantity of antigen; those insensitive to the cytotoxic antibody had fewer antigenic determinants. Peripheral blood leukocytes also contained the antigen in low concentration. Absorption experiments were performed with autochthonous leukocytes to rule out the possibility that isoantibodies were contributing to the cytotoxic activity. The individual's own leukocytes could absorb the cytotoxic activity from his own serum. Antigenic activity was also demonstrated in spleen, bone marrow, liver, kidney, and brain. Erythrocytes had no detectable antigenic activity. These studies indicate that the antigen is neither organ-specific nor tumor-specific.

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