Immunocytological localization of estrogen in human mammary carcinoma cells by horseradish—anti-horseradish peroxidase complex

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Abstract
Tissues from 25 female and 2 male patients of mammary carcinoma were examined by the horseradish—anti‐horseradish peroxidase complex to localize the presence of estrogen. Carcinoma cells of 10 female and 2 male patients showed positive staining by this technique. The nucleus and the cytoplasmic membrane showed the reaction. This method is useful in identifying the exact location of the hormones or hormone‐like substances in the malignant cells and, perhaps, will be of great help in selecting the patients for management.