Immunological cross‐reactivity between the light‐harvesting chlorophylla/b‐proteins of a marine green alga and spinach
- 1 August 1987
- journal article
- research article
- Published by Wiley in Physiologia Plantarum
- Vol. 70 (4) , 597-602
- https://doi.org/10.1111/j.1399-3054.1987.tb04311.x
Abstract
Immunoblotting was used to probe the reactivity of rabbit polyclonal antibodies against PS1I and PSI light‐harvesting chlorophyll a/b‐proteins of spinach (Spinacea oleraceaL.) with the light‐harvesting complexes of a siphonaceous marine alga,Codium, that have more chlorophyll b, siphonaxanthin and siphonein instead of the lutein. The spinach LHCII antibodies cross‐reacted only with the apoproteins of Cod‐ium LHCII. Antisera against the spinach LHCI apoproteins showed strong affinity for the apoproteins ofCodiumLHCI, and also reacted with the polypeptides of spinach LHCII and Codium LHCII. Our results indicate some similarities in the amino acid sequences between theCodiumsiphonaxanthin‐Chl a/fe‐proteins of LHCII and LHCI and the corresponding spinach lutein‐chlorophyll a/b‐proteins.Keywords
This publication has 21 references indexed in Scilit:
- The chlorophylla/b‐proteins of PS I and PS II are immunologically relatedFEBS Letters, 1986
- Monoclonal antibodies specific for the light‐harvesting chlorophylla/b‐protein complex (LHC)FEBS Letters, 1985
- Isolation and characterization of a siphonaxanthin-chlorophyll ab-protein complex of Photosystem I from a Codium species (Siphonales)Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Chlorophyll-protein complexes of a marine green alga, Codium species (Siphonales)Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- The chlorophyll-protein complexes of Prochloron sp. (Prochlorophyta)Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Thylakoid polypeptide composition and light-independent phosphorylation of the chlorophyll a,b-protein in Prochloron, a prokaryote exhibiting oxygenic photosynthesisBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984
- Studies of chloroplast thylakoid proteins using monoclonal antibodiesBiochemical and Biophysical Research Communications, 1984
- COMPARATIVE BIOCHEMISTRY OF PHOTOSYNTHETIC LIGHT-HARVESTING SYSTEMSAnnual Review of Biochemistry, 1983
- The Role of the Light‐Harvesting Chlorophyll a/b ‐Protein Complex in Chloroplast Membrane StackingEuropean Journal of Biochemistry, 1980
- The light-harvesting chlorophyll a/b · protein complex of the green alga Acetabularia mediterranea isolation and characterization of two subunitsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977