Measurement of the Enzyme-CO2-Mg2+ Form of Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase
- 1 February 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 80 (2) , 599-600
- https://doi.org/10.1104/pp.80.2.599
Abstract
When the amount of activation of ribulose 1,5-bisphosphate carboxylase has been measured, two forms of the enzyme, not one, are actually determined experimentally. Only the enzyme-activator, CO2-Mg2+ form can bind ribulose bisphosphate for reaction with substrate CO2 or O2. A method is presented which measures only this catalytically active form by stabilizing it with ribulose biphosphate just before dilution and assay in Mg2+-free reaction medium.This publication has 6 references indexed in Scilit:
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