Separation of protoporphyrins and related compounds by reversed-phase liquid chromatography.
Open Access
- 1 April 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (4) , 605-610
- https://doi.org/10.1093/clinchem/25.4.605
Abstract
We describe the separation of protoporphyrin and related porphyrins by reversed-phase "high-performance" liquid chromatography, with fluorometric detection. We used the method to demonstrate that acid hydrolysis of the dimethyl ester of protoporphyrin IX is complete in 2 to 3 h and is followed by the acid-catalyzed conversion of protoporphyrin IX to a chemical species that chromatographic evidence indicates to be hematoporphyrin IX. In addition, the method was used to evaluate the purity of a commercial preparation of protoporphyrin IX and was also demonstrated to have the sensitivity and specificity needed for measuring the protoporphyrin IX content of whole-blood.This publication has 3 references indexed in Scilit:
- A new rapid method for isolation of naturally occurring porphyrins and their quantitation after high performance liquid chromatographyAnalytical Biochemistry, 1978
- The differentiation of the porphyrias by means of high pressure liquid chromatographyClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Separation of Porphyrins by means of Counter-Current DistributionScandinavian Journal of Clinical and Laboratory Investigation, 1953