Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
- 1 July 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (7) , 4147-4151
- https://doi.org/10.1073/pnas.77.7.4147
Abstract
The expression of cloned eukaryotic tRNA genes was studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: on tRNA transcription is observed when DNA synthesis is inhibited. Single-stranded DNA apparently does not serve as a template for faithful transcription of this gene in injected oocytes.This publication has 15 references indexed in Scilit:
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