Histological Demonstration of Capillaries, Interstitial Space and Muscle Fibers in Heart and Skeletal Muscle with Fluorescent Dyes
- 1 January 1983
- journal article
- research article
- Published by S. Karger AG in Cells Tissues Organs
- Vol. 116 (4) , 285-289
- https://doi.org/10.1159/000145753
Abstract
A method is described which allows a clear demonstration of capillaries and muscle fibers in the heart and skeletal muscle of experimental animals. The fluorescent dyes fluorescein isothiocyanate (FITC) and lissamine rhodamine B 200 (RB 200) were conjugated with a protein of high (γ-globulin) and low (myoglobin) molecular weight, respectively, and were intravitally injected into the vascular system of rats. FITC globulin distributes itself in the intravasal space and RB 200 myoglobin in the extracellular. In histological sections the capillary lumina and the borderlines of the muscular fibers can be clearly identified and quantitatively evaluated because of the selective fluorescence in the respective structures.Keywords
This publication has 1 reference indexed in Scilit:
- Arterial blood gases, muscle fiber diameter and intercapillary distance in cardiac hypertrophy of rats with an old myocardial infarctionPflügers Archiv - European Journal of Physiology, 1978