Inhibition of rabbit tissue kininase by anti-(endo-oligopeptidase A) antibodies

Abstract
Highly purified rabbit brain endo-oligopeptidase A injected into goats produced, after 60 days of immunization, antisera that specifically inhibit purified rabbit brain endooligopeptidase A. An immunoreactive kiniase having the same specificity as rabbit brain endo-oligopeptidase A for bradykinin was detected in several rabbit tissues. The highest amount of this immunoreactive kininase was found in the 25,000 g supernatant fraction (S fraction) of heart, liver, skeletal muscle, ovary, brain and testis homogenates, corresponding to 89, 86, 78, 59, 56 and 53%, respectively, of the whole kininase activity found in the S fraction.