Effect of Monolayer Surface Pressure on the Activities of Phosphoinositide-Specific Phospholipase C-.beta.1, -.gamma.1, and -.delta.1

Publisher Website
Google Scholar
Abstract
Three isoforms of phospholipase C, either PLC-beta(1), PLC-gamma(1), or PLC-delta(1), were added to the aqueous subphase beneath phospholipid monolayers formed at an air-solution interface, and the initial rate of hydrolysis of phosphatidylinositol 4,5-bisphosphate was measured after addition of 10 mu M free Ca2+. The monolayers were formed from mixtures of phosphatidylcholine (65% PC), phosphatidylserine (33% PS), and phosphatidylinositol 4,5-bisphosphate (2% PIP2). Increasing the surface pressure of the monolayer, pi, from 15 to 25 mN/m decreases the rate of hydrolysis 16-, 13-, and 5-fold for PLC-beta(1), PLC-gamma(1), and PLC-delta(1), respectively. The simplest interpretation of these results is that a portion of each of the enzymes of area A(p) must insert into the monolayer, doing work pi A(p), prior to hydrolysis of PIP2; binding studies with simple model compounds of known cross-sectional area are consistent with this interpretation. Removing the monovalent acidic lipid PS from the monolayer decreases the initial rates of hydrolysis of PIP2 about 3-fold for each PLC isoform, which suggests that negative electrostatic surface potentials increase the PLC activity.