Genetic knockouts in mice: An update
- 1 November 1995
- journal article
- review article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 51 (11) , 1028-1039
- https://doi.org/10.1007/bf01946911
Abstract
Gene disruption technology in mammals, by homologous recombination in embryonic stem cells, is a powerful method to manipulate the mouse germ line. In the past decade it has produced a wealth of knowledge concerning neuronal development, neurodegenerative disorders and the roles of oncogenes, Hox genes and growth factors during development. A surprising variety of genes, however, have given unexpected and disappointing results. A gene/function redundancy theory proposed by many investigators to explain the unexpected results has been supported in certain cases by the generation of double knockout mice. Modification of the basic technology now allows the investigators to carry out a variety of manipulations including conditional or tissue-specific knockout. This may provide a better opportunity in the future for the gene therapy approach to correct the genetic disorder.Keywords
This publication has 139 references indexed in Scilit:
- P-selectin/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli.Journal of Clinical Investigation, 1995
- MARCKS deficiency in mice leads to abnormal brain development and perinatal death.Proceedings of the National Academy of Sciences, 1995
- Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prionsNeuron, 1995
- Targeted mutation in the col5a2 gene reveals a regulatory role for type V collagen during matrix assemblyNature Genetics, 1995
- Insulin-promoter-factor 1 is required for pancreas development in miceNature, 1994
- Gene targeting and synaptic plasticityCurrent Opinion in Neurobiology, 1994
- Endothelial NOS and the Blockade of LTP by NOS Inhibitors in Mice Lacking Neuronal NOSScience, 1994
- Whisker-related neuronal patterns fail to develop in the trigeminal brainstem nuclei of NMDAR1 knockout micePublished by Elsevier ,1994
- Animal models of human genetic diseasesTrends in Genetics, 1993
- Gene disruption in mammalsCurrent Opinion in Genetics & Development, 1991