Determination of Spectinomycin Dihydrochloride by Liquid Chromatography with Electrochemical Detection
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Pharmaceutical Research
- Vol. 05 (10) , 664-667
- https://doi.org/10.1023/a:1015983122499
Abstract
Spectinomycin dihydrochloride is determined by liquid chromatography with electrochemical detection. The drug is chromatographed on a reverse-phase Nucleosil C18 column using an eluent containing 0.02 M sodium citrate and 0.0015 M octyl sodium sulfate (pH 6.10 with perchloric acid) and acetonitrile (100:4). Detection is performed using a coulometric detector (porous carbon working electrode) at + 0.85 V. The drug and primary degradation product are detectable. Detector response is linear to at least 20 µg/ml, which is four times the assay level. The procedure has relative standard deviations of ± 1.21 to ± 2.72% for three lots of bulk drug. Sensitivity is greater than 0.1 µg/ml of spectinomycin (5 ng on column). Repeatability at this level is ±4.94%.This publication has 6 references indexed in Scilit:
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