The growth of African horse-sickness virus in embryonated hen eggs and the transmission of virus byCulicoides variipennis Coquillett (Diptera, Ceratopogonidae)

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Abstract
Summary Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos.Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26°–27° C transmitted African Horse Sickness virus to uninfected eggs.C. variipennis may therefore be considered a biological vector of African Horse Sickness virus in the laboratory.