Induction of Division Synchrony in Tetrahymena pyriformis by a Single Hypoxic Shock. Its Use in Elucidating Control of the Cell Cycle by Adenosine 3': 5'-Monophosphate

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Abstract
A single hypoxic shock was used to induced division synchrony in T. pyriformis. Hypoxia results in accumulation of the cells in the G2 phase of the cell cycle. Cyclic[c]AMP and adenylate cyclase activity were measured in this system. Cell-cycle blockade was associated with extraordinarily high [EC 4.6.1.1] levels of intracellular cAMP. After release from hypoxia, the cells retained characteristic pattern of modulation of cAMP associated with division found in selection-synchronized cells. Results are discussed with reference to other methods of induction synchrony and related studies on the natural cell cycle in this organism.