Development of calcium currents in cultures of mouse spinal cord and dorsal root ganglion neurones

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Abstract
CULTURED spinal cord (SC) and dorsal root ganglion (DRG) neurones of 11–13 day old foetal mice were investigated electro-physiologically during differentiation in vitro using the whole-cell patch-clamp technique. High-voltage-activated calcium currents (HVA) and low-voltage-activated (LVA) calcium currents were measured using barium ions as charge carrier. During differentiation in vitro the soma diameter of SC-neurones increased with age (0–42 days in vitro) from 10.3 ± 2.7 μm to 25.1 ± 5.9 μm. The capacitance of the soma increased from 7.4 ± 2.3 pF to 34 ± 6 pF. The inward calcium current amplitudes increased from 200 pA to 3 nA, while the LVA current amplitude increased only from 50 pA to 100–150 pA. The currents per membrane area through HVA calcium channels increased in the investigated time while the currents through LVA channels decreased.

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