E.S.R. of Spin-trapped Radicals in γ-irradiated Polycrystalline Amino Acids. Chromatographic Separation of Radicals

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Abstract
The free radicals produced by .gamma.-radiolysis of polycrystalline amino acids (L-valine, L-leucine, L-isoleucine and L-proline) at room temperature in the absence of air were investigated by spin trapping with 2-methyl-2-nitrosopropane (MNP). The spin adducts produced by dissolving the irradiated solids in aqueous MNP solutions were separated by high-performance liquid chromatography and then identified by ESR. Deamination (ring-opening reaction for L-proline) was observed for all amino acids. For L-valine and L-leucine, H-abstraction from the tertiary C in the side chains occurred. For isoleucine, H-abstractions from the .alpha.-carbon of the amino acid and from a non-terminal C in the side chain were found.