FORMATION OF AN EPINEPHRIN-LIKE SUBSTANCE IN AUTOLYZING ADRENAL GLANDS

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Abstract
Autolysis of crushed, whole adrenal glands gives rise to a powerful pressor substance which may be obtained by dialysis. This substance gives ail the ordinary chemical tests for adrenalin, but differs from it in that it is not precipltated by conc. NH40H. It does not give a biuret reaction; is not destroyed by boiling for 1 min.; is adsorbed by animal charcoal and aluminum hydroxide and does not lose its physiological effect if exposed to air at room temp.for 30 days. Glacial acetic acid does not destroy its pressor principle. Inhibiting autolysis of the crushed adrenal glands by the addition of KCN prevents the formation of the pressor substance as detd. by chemical and physiological tests. The pressor substance contained in the dialysate is not precipltated by conc. NH4OH, although a heavy flocculent precipltate forms. The mother liquor from this precipltate contains a powerful pressor substance. The formed pressor substance may be removed from the crushed adrenal glands by repeated washing; further autolysis results in the formation of more pressor substance, which continues to form until the gland substance is practically completely disintegrated.

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