EQUINE RHINOPNEUMONITIS VIRUS (HERPESVIRUS TYPE-1) - ATTENUATION IN STABLE MONKEY CELL LINE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (8) , 1211-1215
Abstract
An isolate of virulent equine herpesvirus (EHV) type 1 was adapted to Vero [green monkey kidney] stable cell line by 13 serial passages at 37.degree. C and 50 serial passages at 26.degree. C. The attenuated EHV-1 were avirulent, but immunogenic in horses if injected i.m. The attenuated virus was regularly isolated from peripheral leukocytes in inoculated horses, but was not recovered from nasal turbinate tissues. A mild leukopenia was noticed. The attenuated virus produced characteristic large syncytia on primary isolation in rabbit kidney (RK13) or Vero cells at 37.degree. C in contrast to cell rounding observed with virulent EHV-1. The syncytical marker was stable through 20 serial passages in Vero cells at 37.degree. C. New application of double immunodiffusion test for distinguishing between EHV-1 and EHV-2 also is described.

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