Continuous Production of Carcinoembryonic Antigen in Hollow Fiber Cell Culture Units: Brief Communication 2 3
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 60 (2) , 303-306
- https://doi.org/10.1093/jnci/60.2.303
Abstract
A line of cultured cells derived from a primary human adenocarcinoma of the colon was grown in the extracapillary spaces of hollow fiber tissue culture units in the absence of serum components of high molecular weight (>10,000). The cells were capable of producing more than 30 μg of carcinoembryonic antigen (CEA) per day, provided that the extracapillary fluid was changed frequently. The concanavalin-A binding and molecular sieve chromatography properties of the tissue culture-derived CEA were similar to those of CEA isolated from metastatic colon cancer tissue.Keywords
This publication has 1 reference indexed in Scilit:
- Human Colon Adenocarcinoma Cells. II. Tumorigenic and Organoid Expression In Vivo and In Vitro2JNCI Journal of the National Cancer Institute, 1977