Solution conformations of B. subtilis ribosomal 5S RNA: A calorimetric study
- 1 July 1986
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 25 (7) , 1299-1313
- https://doi.org/10.1002/bip.360250710
Abstract
The unfolding of B. subtilis 5S RNA is examined by direct calorimetric measurement in the presence of various concentrations of Na+ and Mg2+. The composite differential scanning calorimetry (DSC) curve is analyzed into 3–5 individual two‐state melting transitions. In the absence of added Na+ or Mg2+, the 5S RNA segments melt together at Tm = 40°C. Addition of Na+ stabilizes the molecular structure (Tm = 56°C) and widens the melting temperature range, so that up to five component transitions are observed. Addition of Mg2+ alone produces a very stable structure (Tm = 75°C) with highly cooperative melting. Finally, addition of both Na+ and Mg2+ produces the highest stability (Tm = 76°C). The results are interpreted according to hypothetical secondary and tertiary base‐pairing schemes. The conformational changes demonstrated here may facilitate the movement of the protein synthesis machinery during RNA translation.This publication has 41 references indexed in Scilit:
- Assignment of resonances of exchangeable protons in the NMR spectrum of the complex formed by Escherichia coli ribosomal protein L25 and uniformly nitrogen-15 enriched 5 S RNA fragmentFEBS Letters, 1984
- Conservation of secondary structure in 5 S ribosomal RNA : a uniform model for eukaryotic, eubacterial, archaebacterial and organelle sequences is energetically favourableBiochimie, 1982
- A general model for the conformational switch in 5S RNA during protein synthesisJournal of Theoretical Biology, 1980
- Lasar raman evidence for a new cloverleaf secondary structure for eucaryotic 5 S RNAJournal of Molecular Biology, 1978
- Thermodynamic analysis of transfer RNA unfoldingJournal of Molecular Biology, 1978
- A new method for the isolation of A 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomesFEBS Letters, 1977
- The architecture of 5S rRNA and its relation to functionJournal of Molecular Evolution, 1975
- The nucleotide sequence of the 5S ribosomal RNA from a photobacteriumJournal of Molecular Evolution, 1975
- A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric studyJournal of Molecular Biology, 1974
- Free energy of imperfect nucleic acid helices: II. Small Hairpin LoopsJournal of Molecular Biology, 1973