Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs.
- 1 July 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (14) , 5057-5061
- https://doi.org/10.1073/pnas.83.14.5057
Abstract
The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein specifically binds to DNA regions containing a single base-pair mismatch. The protein displayed variable affinity for the limited set of mismatches tested (G-T > G-A .apprxeq. A-C > T-C).Keywords
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