On-the-Fly Fluorescence Lifetime Determination with Total Emission Detection in HPLC
- 1 September 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (17) , 3458-3464
- https://doi.org/10.1021/ac961095j
Abstract
The entire fluorescence decay profile during HPLC elution has been directly measured on-the-fly in HPLC at higher sensitivity than in previous literature reports. The fluorescence is excited with the fourth harmonic (266 nm) of a pulsed Nd:YAG laser system and detected broadband with a photomultiplier tube and a digital storage oscilloscope. Detection limits in the range 1-10 ppb are found for several individual polycyclic aromatic hydrocarbons (PAHs) when the total time-integrated fluorescence is analyzed. The chromatograms of PAH mixtures containing 8-10 species were lifetime analyzed with a simple phase plane analysis, in which a single lifetime is determined from the fluorescence decay profile for each point on the chromatogram. The determination of lifetimes under coelution conditions is also illustrated and discussed.Keywords
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