Periodic Polypeptides Based on Poly(L-Alanylglycine): Biological Synthesis and Verification of the Structure of A Series of Polymers Containing Tandem —(Alagly)xGlugly— Repeats

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Abstract
The experiments reported herein constitute part of an investigation of the relationship between primary sequence and higher order structure in repetitive polypeptides. Three series of artificial DNAs encoding poly-peptides represented by the general formula + (GlyAla)nGlyGlu]m— were constructed and cloned in Escherichia coli.Protein expression was monitored by in-vivo labeling with 3H-glycine. Protein products were isolated from the cell lysates by stepwise acidification in yields of 20–50 mg/L of culture, and fusion fragments derived from the cloning vectors were removed by cyanogen bromide cleavage. Amino acid sequence analysis verified the expected sequences through the first 40–50 N-terminal residues.