Bovine γ‐Aminobutyric AcidA Receptor Sequence‐Specific Antibodies: Identification of Two Epitopes Which Are Recognised in Both Native and Denatured γ‐Aminobutyric AcidA Receptors
- 1 July 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 53 (1) , 132-139
- https://doi.org/10.1111/j.1471-4159.1989.tb07304.x
Abstract
Polyclonal antibodies have been raised against synthetic peptides whose sequences correspond to the N-terminal 15 amino acids and the C-terminal 17 amino acids of the bovine .gamma.-aminobutyric acidA (GABAA) receptor .alpha.1 subunit. These antibodies were shown to react with the denatured GABAA receptor .alpha. subunit, Mr 53,000, in Western blots with both purified receptor and brain membranes as antigens. Also, both antibodies recognised both the purified and detergent-solubilised GABAA receptor as demonstrated by dose-dependent specific immunoprecipitation of the GABA and benzodiazepine binding sites from solution. Evidence is also presented to show brain-regional distribution of the expression of the .alpha.1 subunit.Keywords
This publication has 28 references indexed in Scilit:
- Molecular Size of the ?-Aminobutyric AcidAReceptor Purified from Mammalian Cerebral CortexJournal of Neurochemistry, 1989
- Distinct GABAA receptor α subunit mRNAs show differential patterns of expression in bovine brainNeuron, 1988
- Structural and functional basis for GABAA receptor heterogeneityNature, 1988
- The γ‐aminobutyric‐acid/benzodiazepine‐receptor protein from rat brainEuropean Journal of Biochemistry, 1987
- Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-familyNature, 1987
- The type I and type II γ-aminobutyric acid/benzodiazepine receptor: 1. Purification and two-dimensional electrophoretic analysis of the receptor from cortex and cerebellumBiochemical and Biophysical Research Communications, 1987
- [3H]Muscimol photolabels the γ-aminobutyric acid receptor binding site on a peptide subunit distinct from that labeled with benzodiazepinesBiochemical and Biophysical Research Communications, 1986
- Antibodies Recognising the GABAA/Benzodiazepine Receptor Including Its Regulatory SitesJournal of Neurochemistry, 1986
- Physicochemical Characterization of Detergent‐Solubilized γ‐Aminobutyric Acid and Benzodiazepine Receptor Proteins from Bovine BrainEuropean Journal of Biochemistry, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981