Studies on the proton‐translocating NADH:ubiquinone oxidoreductases of mitochondria and Escherichia coli using the inhibitor 1,10‐phenanthroline
- 14 February 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 339 (1-2) , 142-146
- https://doi.org/10.1016/0014-5793(94)80402-8
Abstract
Mitochondrial NADH:ubiquinone oxidoreductase (complex I) is uncompetitively inhibited by 1,10‐phenanthroline (OP). EPR spectroscopy of submitochondrial particles indicates that OP, similarly to rotenone, inhibits electron transfer between the Fe‐S clusters of complex I and the ubiquinone pool. The proton‐translocating NADH dehydrogenase (NDH1) of E. coli is more sensitive to OP than is NDH1 of Paracoccus. EPR spectroscopy of membranous E. coli NDH1 shows that two slow‐ and one fast‐relaxing Fe‐S clusters become detectable upon reduction by NADH in the presence of OP. However, none of them resembles the mitochondrial cluster 2.Keywords
This publication has 20 references indexed in Scilit:
- The Gene Locus of the Proton-translocating NADH : Ubiquinone Oxidoreductase in Escherichia coliJournal of Molecular Biology, 1993
- Demonstration of separate genetic loci encoding distinct membrane-bound respiratory NADH dehydrogenases in Escherichia coliJournal of Bacteriology, 1993
- The bacterial energy-transducing NADH-quinone oxidoreductasesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1993
- Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organismsArchives of Biochemistry and Biophysics, 1990
- Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNAGene, 1990
- Characterization of four herbicide-resistant mutants of Rhodopseudomonas viridis by genetic analysis, electron paramagnetic resonance, and optical spectroscopyBiochemistry, 1989
- Inhibition of mitochondrial electron transport by hydrophilic metal chelators. Determination of dehydrogenase topographyBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- Low molecular weight analogs of coenzyme Q as hydrogen acceptors and donors in systems of the respiratory chainBiochemical and Biophysical Research Communications, 1975
- Quinone interaction with the respiratory chain-linked NADH dehydrogenase of beef heart mitochondria. II. Duroquinone reductase activityBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1971
- SPECTRAL EVIDENCE FOR A NEW PHOTOREACTIVE COMPONENT OF THE OXYGEN-EVOLVING SYSTEM IN PHOTOSYNTHESISProceedings of the National Academy of Sciences, 1969