Phagocytosis of Opsonized Fluorescent Microspheres by Equine Polymorphonuclear Leukocytes
- 12 January 1990
- journal article
- research article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 37 (1-10) , 481-490
- https://doi.org/10.1111/j.1439-0450.1990.tb01087.x
Abstract
Equine blood polymorphonuclear leukocytes (PMN) were isolated by buffy coat and hypotonic lysis of residual erythrocytes. A highly reproducible method is described for measuring the uptake of opsonized latex microspheres by equine PMN using flowcytometry. The use of cytochalasin D allowed for differentiation of ingested from attached particles. The kinetics of phagocytosis in vitro is shown for different experimental conditions. We developed an assay for evaluation of phagocytic capacity of PMN which allows the assessment of drugs for their influence on phagocytosis in vivo as well as in vitro.This publication has 36 references indexed in Scilit:
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