Nucleotide Sequence and Expression of the Diphtheria tox 228 Gene in Escherichia coli

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Abstract
The complete nucleotide sequence of the diphtheria tox 228 gene encoding the nontoxic serologically related protein CRM228 has been determined. A comparison of the predicted amino acid sequence with the available amino acid sequences from the wild-type toxin made it possible to deduce essentially the entire nucleotide sequence of the wild-type tox gene. The signal peptide of pro-diphtheria toxin and the putative tox promoter have been identified, a highly symmetrical nucleotide sequence downstream of the toxin gene has been detected; this region may be the corynebacteriophage β attachment site ( att P). The cloned toxin gene was expressed at a low level in Escherichia coli .