BIOSYNTHESIS OF CITRIC ACID
- 1 August 1951
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 62 (2) , 235-238
- https://doi.org/10.1128/jb.62.2.235-238.1951
Abstract
Labeled citric acid was synthesized by Aspergillus niger from sucrose in the presence of Na ace-tate-2-C14 and C13O2. The labeled citric acid was degraded by a new method which allowed a separate isotope detn. of each of the 6 C atoms. The finding of wide divergence in labeling between the primary carboxyls confirmed the Ogston concept in the case of a microbial system. The exptl. evidence indicated extensive C4- or C6-acid cycling in an organism which has a C metabolism predominantly shifted toward the bulk production and excretion of citric acid.Keywords
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