Detection of urinary metabolites of 2‐acetylaminofuorene by cultured hamster embryo cells and high performance liquid chromatography
- 1 January 1981
- journal article
- Published by Wiley in Teratogenesis, Carcinogenesis, and Mutagenesis
- Vol. 1 (2) , 235-244
- https://doi.org/10.1002/tcm.1770010211
Abstract
Urine was collected from Sprague‐Dawley rats injected intraperitoneally with a suspension of 2‐acetylaminofluorene (AAF) or with an inert suspension vehicle. Morphological transformation was observed in clonal cultures of secondary passage hamster embryo cells (HEC) after the addition of AAF‐treated rat urine or concentrated urine extracts prepared by incubating the urine with β‐glucuronidase. No morphological alteration was observed in cultures treated with urine (or urine extracts) from rats injected with the suspension vehicle alone. The presence of N‐hydroxy‐ and ring‐hydroxylated metabolites of AAF in the AAF‐treated concentrated urine extract was confirmed by highperformance liquid chromatography (HPLC).Keywords
This publication has 19 references indexed in Scilit:
- MUTAGENICITY IN URINE OF NURSES HANDLING CYTOSTATIC DRUGSThe Lancet, 1979
- In vitro transformation of hamster embryo cells by betel tobacco extractsToxicology Letters, 1978
- Statistical evaluation of Pienta's in vitro carcinogenesis assayToxicology Letters, 1978
- An Analytical Approach to Metabolic Profiling of Aromatic Compounds Using Liquid ChromatographyJournal of Liquid Chromatography, 1978
- Morphological transformation of early passage Golden Syrian Hamster embryo cells derived from cryopreserved primary cultures as a reliable in vitro bioassay for identifying diverse carcinogensInternational Journal of Cancer, 1977
- Determination of 2-acetylaminofluorene and its metabolites in urine by high pressure liquid chromatographyBiochemical Medicine, 1976
- Detection of metabolic carcinogen intermediates in urine of carcinogen-fed rats by means of bacterial mutagenesisNature, 1974
- Cyclophosphamide: Pilot study of genetically active metabolites in the urine of a treated human patient induction of mitotic gene conversions in yeastMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- In vitro Transformation of Syrian Hamster Embryo Cells by Diverse Chemical CarcinogensNature, 1972
- In Vitro Cell Transformation with Chemical CarcinogensNature, 1963