Selenium in Bovine Spermatozoa

Abstract
The association of Se with ejaculated bovine spermatozoa was investigated. Over 75% of the radioactive 75 Se was released after 30 min of incubation in 2 .times. 10-3 dithiothreitol. Of the 75 Se released by dithiothreitol, 85% was associated with spermatozoal protein. Protein containing 75 Se was found predominantly in a single band after polyacrylamide gel electrophoresis. MW was .apprx. 21,500 daltons.