METHODS OF IMPREGNATING NEUROFIBRILLAR SUBSTANCE ON SLIDES AFTER IMBEDDING

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Abstract
Imbedded and sectioned material may be impregnated for demonstration of neurofibrillae by any of three new methods. The direct albumen method uses 0.2 - 0.5% egg albumen and 2% AgNO3. Slides are kept in the Ag colloid bath for 5 days at 37[degree]C or for 8 days at room temp. They are toned for 10 min. in 0.2% aqueous AuCl3 soln. and reduced in 0.2% aqueous oxalic acid soln. for 1 hr. There is intermediate washing in distilled water. The direct protargol (Au) method uses a bath which is 2% aqueous protargol. The indirect albumen (Ag-Au) method is used to supplement the direct albumen method when results are unsatisfactory. The time in the bath is shorter and the subsequent washing short and critical. When the section is reduced by a Na sulfite-Na bisulfite buffered amidol soln. with pH 6.5-7.5 made from 5 parts stock, 10% aqueous Na sulfite and 1 part 5% aqueous Na bisulfite in 1% amidol. After dropping on slide it reduces in 30-60 sec. and is then washed completely. The remaining steps are the same. The direct albumen method is simplest, most constant and most uniform.

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