Sporulation of the FungusHirsutella rhossiliensisfrom the NematodeCriconemella xenoplax

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Abstract
H. rhossiliensis emerged from colonized C. xenoplax when incubated for 1 day at 25.degree. C in moist chambers or soil. Emergent hyphae branched infrequently and developed phialides and spores after 2-3 days. With the host nematode as the only substrate, about 700 spores/nematode were produced in moist chambers. Sporulation ceased after 21 days. Optimum, maximum and minimum temperatures for sporulation from colonized nematodes on agarose were about 25.degree., 30.degree. and 10.degree. C, respectively. Spore production was generally high at pH 5.5-7.0, the range common in South Carolina [USA] peach orchard soils. Sporulation on agarose was not stimulated by KCl or NaCl. Decreasing the osmotic potential below -5 bars with these salts suppressed spore production until few or no spores were observed at -40 bars.

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