Optimal Activation of Isopsoralen To Prevent Amplicon Carryover
- 1 January 1999
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 37 (1) , 261-262
- https://doi.org/10.1128/jcm.37.1.261-262.1999
Abstract
We compared the efficiencies of activation of the photochemical isopsoralen compound 10 and its resulting amplicon neutralizations under conditions with a UV transilluminator box at room temperature (RT) and a HRI-300 UV photothermal reaction chamber at RT and at 5°C. Our data suggest that use of the HRI-300 reaction chamber at 5°C results in a statistically significantly higher degree of amplicon neutralization.Keywords
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