Purification and some properties of oat golden stripe virus

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Abstract
SUMMARY: Oat golden stripe virus (OGSV) was maintained in oats by mechanical inoculation and purified by extraction of leaves in borate buffer, two cycles of centrifugation through sucrose cushions and isopycnic centrifugation in CsCl. An antiserum with a titre of 1/1024 in precipitin tests was prepared. Particle length distribution was bimodal with median values, respectively, of 150 and 300 nm from dip preparations. Measurements from immunosorbent electron microscopy (ISEM) and purified preparations showed that the particles had partially degraded during these procedures. The virus sedimented as two components of 168 S and 218 S and had a buoyant density of 1321 g cm‐3.Four isolates of OGSV reacted with the antiserum. Antiserum to members and possible members of the furovirus group were tested in ISEM decoration tests and in ELISA. OGSV was related to soil‐borne wheat mosaic virus but not to beet necrotic yellow vein virus, hypochoeris mosaic virus or potato mop‐top virus.