Reduction of disulphide bonds in proteins mixed disulphides catalysed by a thioltransferase in rat liver cytosol
- 1 September 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 149 (3) , 785-788
- https://doi.org/10.1042/bj1490785
Abstract
The reduction of mixed disulphides of some proteins and GSH [Protein(-SSG)n] is accomplished with GSH as a reductant and a thioltransferase from rat liver as a catalyst, thus: See article. The spontaneous reaction is negligible in comparison with the enzymic reaction in vivo, and any direct reduction with glutathione reductase is not detectable with the substrates used. The reduction is only indirectly dependent on NADPH, which is required for the regeneration of GSH from GSSG. Other protein disulphides apparently are reduced via analogous GSH-dependent reactionsKeywords
This publication has 15 references indexed in Scilit:
- Synthesis of a mixed disulfide of egg white lysozyme and glutathione - a model substrate for enzymatic reduction of protein mixed disulfidesFEBS Letters, 1975
- Enzymatic catalysis of the reversible sulfitolysis of glutathione disulfide and the biological reduction of thiosulfate estersArchives of Biochemistry and Biophysics, 1974
- The nature of the enzymatic reduction of the mixed disulfide of coenzyme A and glutathioneFEBS Letters, 1974
- Mechanism of action of enzymes catalyzing thiol—disulfide interchange. Thioltransferases rather than transhydrogenasesFEBS Letters, 1974
- Resolution of Glutathione-linked Enzymes in Rat Liver and Evaluation of their Contribution to Disulfide Reduction via Thiol-Disulfide Interchange.Acta Chemica Scandinavica, 1974
- Comparison of human hemoglobin A carrying glutathione and a mixed disulfide with the naturally occurring human hemoglobin A3Biochemistry, 1973
- The occurrence of protein-bound mixed disulfides in rat tissuesBiochimica et Biophysica Acta (BBA) - Protein Structure, 1973
- Release of Thiols from Cellular Mixed Disulphides and Its Possible Role in Radiation ProtectionInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1972
- The reduction of the L‐cysteine‐glutathione mixed disulfide in rat liver. involvement of an enzyme catalyzing thiol‐disulfide interchangeFEBS Letters, 1970
- Cellular mixed disulphides between thiols and proteins, and their possible implication for radiation protectionBiochemical Pharmacology, 1968