Effect of Phosphates on the Structure of the Actin Filament1

1 November 1975

journal article
research article
Published by Oxford University Press (OUP) in The Journal of Biochemistry

Vol. 78 (5) , 1101-1104
https://doi.org/10.1093/oxfordjournals.jbchem.a130988

Abstract

The fine structure of the purified actin filament was investigated by negative staining. The actin filament polymerized in Tris-HCl buffer and KCl showed a collapsed image different from that of a double stranded helix. Addition of ATP, ADP, or inorganic orthophosphate, however, converted it into a straight filament with typical double strands.

Keywords

ATP
STRUCTURE
FILAMENT
HELIX
BUFFER
ACTIN
TRIS
ADP
KCL
CONVERTED