Abstract
A zero flow method of rapidly determining reflection coefficients of giant algae cells is described. Changes of the osmotic pressure in the outside medium caused a net volume flow through the cell membranes and consequently changes of the hydrostatic pressure inside the cell. By continously measuring the hydrostatic pressure it was possible, to determine the concentration outside the cell at which no volume flow occurs. The reflection coefficients of some non-electrolyts were determined on the membrane of the mediterranean algae Valonia utricularis with an error of 3 - 4% and are discussed on the basis of the pore model. The hydrostatic pressure inside Valonia cells decreased exponentially with time, when they were put into a hypertonic aequous solution. If a linear dependence of the volume (water) flow on the hydrostatic and osmotic pressure differences is supposed, it will be possible to calculate the volume (water) flow.

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